Results of molecular polymorphism analysis of Toxoplasma gondii isolates from free-living animal populations in Poland - Marek Kowalczyk (2027)
The dataset contains data obtained during the Miniatura research project (8th edition). The main goal of the Project was to optimize methods for the detection and genotyping of Toxoplasma gondii and to perform a preliminary assessment of the prevalence and genetic polymorphism of this parasite in populations of free-living animals in Poland. The study included biological samples collected from 230 individuals representing five species of free-living animals (beaver, hare, deer, wild boar, raccoon dog). The dataset provides complete documentation of successive stages of the project, including DNA extraction and assessment of DNA quantity and quality, detection of T. gondii using the B1 marker (nested PCR), genotyping of isolates using the MLST approach (12 markers), analysis of microsatellite markers (15 markers), as well as results and raw files of bioinformatic analyses conducted for the obtained sequences. The data include results of PCR amplification, electrophoretic separation, sequencing, analyses of nucleotide and amino acid polymorphisms, similarity matrices, phylogenetic analyses, genetic distance calculations, and in silico restriction digestion. Each sample was assigned a unique identification code, ensuring full traceability of the material from DNA extraction through bioinformatic analyses to sequence deposition in the GenBank database. A central metadata registry contains information on sample origin, species, tissue type, sample preparation method, and links between data generated at individual stages of the project. The hierarchical folder structure of the repository reflects the workflow of the study. The dataset constitutes a comprehensive resource for the analysis of genetic diversity of T. gondii in free-living animal populations, enables comparisons with reference data deposited in public databases, and may be used in studies on toxoplasmosis epidemiology.
Keywords: genotyping; molecular markers; polymorphism; Toxoplasma gondii; Dziedzina nauk rolniczych: Zootechnika i rybactwo; Dziedzina nauk ścisłych i przyrodniczych: Nauki biologiczne